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Short-chain fatty acids (SCFAs) produced by the microbiota can promote intestinal barrier repair through a variety of mechanisms, including activating inflammasomes and affecting the secretion of interleukin (IL)-18, promoting epithelial cell migration, regulating tight junction protein expression, activating G protein-coupled receptors, and inhibiting tissue protein deacetylases [10, 11]

NAPQI readily binds sulfhydryl groups, such as in glutathione (GSH)

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While oral bioavailability is lower than subcutaneous administration, these studies established that even high oral doses maintained excellent safety profiles, though higher doses (54 mg) were associated with increased gastrointestinal discomfort